The singer was dressed in a curve-hugging red outfit that some viewers think showed off baby bump No. 2.
The singer has been open throughout his career about living with Type 1 diabetes.
David Ortiz, Emmitt Smith, Lisa Leslie & Julius Erving also make appearances in the spot.
"What are you doing here?!" the singer demands of her husband.
The singer was joined by Academy Award-winner Troy Kotsur, who delivered an ASL rendition of "The Star Spangled Banner."
The Abbott Elementary actress is the first Black person to perform the Black national anthem on the field before the Super Bowl.
His wife, three children and friends, including Ellen DeGeneres, gathered to honor the late dancer, choreographer, actor and producer.
The documentary is set to premiere later this month on Weverse and Disney+.
"She could just walk out in sweatpants and sing, and that would be just great," he said of her upcoming Super Bowl performance.