The group went on hiatus last year after member Kyle Gass joked about an assassination attempt on then-candidate Donald Trump.
The concert will also feature Cam, Corey Kent, Carter Faith and more.
Organizers said the all-star event drew more than 50 million viewers across 28 streaming platforms.
The free concert took place at the Rose Bowl in Pasadena, Calif., on Feb. 1.
The benefit concert is set for Feb. 1 at the Rose Bowl in Pasadena, California.
YouTube CEO Neal Mohan also revealed upcoming efforts to aid content creators impacted by the wildfires.
The donation comes from the company's entertainment division, which includes Amazon Music, along with Ring and Whole Foods Market.